(November 16, 2011) One week later and my mushroom cells had covered the entire petri dish! The entire dish looked like a fluffy cotton ball, which means that it is super healthy. I only lost three out of fifty petri dishes to some sort of bacteria, which is apparently really lucky of me. Who knew?
I really got down to business this time. I had to inoculate some jars full of chia seeds (Remember those fast growing chia pets? Yeah, these were the seeds that I was working with!) and some regular bags full of other grain/wheat seeds. Each petri dish can be cut into either ten "pizza slices", which can inoculate ten other seed bags, or it can be cut in half and each half can inoculate a chia jar.
In order to inoculate seed bags I had to carefully cut the media in the petri dishes into "pizza slices", while keeping my scalpel extra sanitized in order to not contaminate the seeds. I scooped them into my prepped bags, and then learned how to use the sealer machine to seal them. I then had to shake them for awhile in order to get all the media evenly distributed throughout the bag. After another employee showed me quickly how to do this, Amanda let me finish another set of bags by myself.
In order to inoculate the chia seeds I had to cut the media in the petri dishes in half. I then mixed the media with some of the liquid/seeds from the chia jar. Yes, I was making a media milkshake. This process was a lot easier, since I was dealing with jars instead of the bags.
Hopefully, by the time I come back from break I will see my mushrooms growing all over the seeds!
Caroline, your entries are wonderful - fully explained, lots of details, and very interesting. Keep up the great effort.
ReplyDeleteOne thing to consider adding: WHY are you doing these activities? What purpose do they serve?